Downregulation of Klotho expression by dehydration.
نویسندگان
چکیده
Klotho, a transmembrane protein, protease, and hormone mainly expressed in renal tissue counteracts aging. Overexpression of Klotho substantially prolongs the life span. Klotho deficiency leads to excessive formation of 1,25(OH)(2)D(3), growth deficit, accelerated aging, and early death. Aging is frequently paralleled by dehydration, which is considered to accelerate the development of age-related disorders. The present study explored the possibility that dehydration influences Klotho expression. Klotho transcript levels were determined by RT-PCR, and Klotho protein abundance was detected by Western blotting in renal tissue from hydrated and 36-h-dehydrated mice as well as in human embryonic kidney (HEK293) cells. Dehydration was followed by a significant decline of renal Klotho transcript levels and protein abundance, accompanied by an increase in plasma osmolarity as well as plasma ADH, aldosterone, and 1,25(OH)(2)D(3) levels. Antidiuretic hormone (ADH; 50 nM) and aldosterone (1 μM) significantly decreased Klotho transcription and protein expression in HEK293 cells. In conclusion, the present observations disclose a powerful effect of dehydration on Klotho expression, an effect at least partially mediated by enhanced release of ADH and aldosterone.
منابع مشابه
Dehydration: a new modulator of klotho expression.
ACCORDING TO THE GREEK MYTHS, life and death of all individuals are in the hands of three sister goddesses: Klotho, Lakhesis, and Atropos. The first spins the thread of life, the second determines the length of the thread, while the last cuts the thread. Following this credence, once a putative agesuppressing gene has been identified, it was named klotho (11). Mice lacking klotho have a syndrom...
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ورودعنوان ژورنال:
- American journal of physiology. Renal physiology
دوره 301 4 شماره
صفحات -
تاریخ انتشار 2011